2006
VEGA 1/3558/06
Combinations of capillary electrophoresis techniques for multidimensional separations of multi-component mixtures of low-molecular and high-molecular substances - Dušan Kaniansky, 2006 - 2008
VEGA 1/3562/06
Miniaturized integrated analytical systems for (ultra)trace analysis based on electro-separations on a chip with coupled-columns - Marián Masár, 2006 - 2008
VEGA 1/3583/06
Combinations of liquid chromatography methods and electro-separation methods for multi-component trace analysis of ionogenic substances in complex samples - Milan Hutta, 2006 - 2008
VEGA 1/03563/06
On-line and off-line combinations of liquid chromatography methods and methods of atomic spectrometry for trace analysis and speciation of organometallic substances in biological and environmental samples - Radoslav Halko, 2006 - 2008
VEGA 1/2466/05
Study and development of new analytical methods for elemental speciation and pre-concentration in environmental samples - Mária Žemberyová, 2005-2007
KEGA 3/4185/06
Textbook and didactic aids for subject "Electro-separation methods of analytical chemistry" for the second level of university education - Dušan Kaniansky, 2006 - 2008
KEGA 3/3116/05
School teaching of basic chemical laboratory techniques and safety at work in laboratory with assistance of visualization and motivation with utilization of possibilities of PC technique and internet - Radoslav Halko, 2005-2006
DAAD 13/2004
Development and study of New Analytical Methods for elemental speciation and preconcentration of metals in environmental samples, Institute of Chemistry, Department of Instrumental Analytics, University Duisburg-Essen, Germany - Mária Žemberyová, 2005-2006
Elaboration and study of new analytical procedures for preconcentration and speciation of Be, Mo, V and Cr in water samples for the determination by atomic spectrometry methods. Inorganic speciation of Mo, V, Cr in water samples in regard to differentiaton of oxidation states, using SPE preconcentration of the species. Study of the determination of bioavailable and specific forms using recommended (BCR) single and sequential procedures.
UK/277/2006
RP-HPLC analysis and characterization of lignins isolated from various woods by Klason method - Róbert Góra, 2006
RP HPLC using stepwise gradients of N, N-dimethylformamide (DMF) in a phosphate buffered (pH 3.00) aqueous mobile phase and a wide-pore (30nm) octadecylsilica column has been aplied to the analysis of a variety of lignins - organocell lignin, dioxane lignin, Björkman lignin and Klason lignins from both softwoods and hardwoods, respectively, in order to demonstrate the usefullness of the approach for their characterization. Tandem combination of spectrophotometric (DAD) and fluorimetric detection was used to get more detailed information on chromatographic behaviour of lignins. The results showed that ten-step gradient can induce distinct features of lignins and humic substances. Combination of good solvating properties of DMF for lignins together with wide pore RP sorbent improves surface interactions of the analytes and suppresses influence of size exclusion effects. Thus it provides reproducibility of characterization profiles and robustness of the method. Calculated reproducibility of retention time of selected peaks was 0.46 % RSD. Repeatability of the data within one week (set of 7 data) was 1.1%RSD. These data are representative also for the other well-shaped peaks of analysed substances.
UK/156/2006
Chip with conductivity detection based highly-precise isotachophoretic determinations of ionogenic substances in biopharmaceutical products - Mária Drusková, 2006
UK/166/2006
Two-dimensional separations combining on-line capillary isotachophoresis with capillary zone electrophoresis- Vladimíra Jánošová, 2006
2005
VEGA 1/2466/05
Study and development of new analytical methods for elemental speciation and pre-concentration in environmental samples - Mária Žemberyová, 2005-2007
VEGA 1/0087/03
Miniaturised electroseparation techniques for trace analysis of ionogenic compounds and biopolymers present in complex ionic matrices - Dušan Kaniansky, 2003-2005
The most important contributions that deal with the use of driving electrodes as designed for the electroseparations on chips (original results as documented by an EU patent). An original electrophoretic technique is based on a combination of electrophoresis-zone technique on a column-switching electrophoresis chip. Significant results that dealt with the electroseparation of proteins on the chips (an original combination of the chip with a conductivity detector as intended for the separation of proteins). A new approach to the separation of proteins based on a combination of isotachophoresis with zone electrophoresis on a column-switching chip was elaborated. A significant contribution, as directed to the use of suitable electrolyte solutions, developed for the various electroseparation techniques. One of the application trends, in the electrophoresis chips, is focused on food analysis as documented by original contributions. An original preparative electroseparation system based on capillary isotachophoresis as intended for the separation and fractionation of proteins was elaborated.
VEGA 1/0061/03
Study of the oxidation in chemical processing of wood through new methods of separation and analysis - Principal Investigator: Svetozár Katuščák, Co-investigators: Dušan Kaniansky and Milan Hutta, 2003-2005
Oxidation processes play a key role in many technologies connected with the processing of the most important renewable raw material - wood. Processes include bleaching, de-lignification, ageing, combustion, recycling and processing of wastes. Improvements in our knowledge on the course of oxidation reactions and products in the chemical processing of wood is limited by the multi-component and complex character of the products of lignin oxidation, cellulose and lingo-cellulose materials (LCM). We propose to attain new information on the course of oxidation, delignification and bleaching through utilizing effective new separation methods in combination with multi-detecting systems. The aim of the project is to utilize previous achievements in the techniques of miniaturized analytical systems (lab-on-a-chip concept), new monolithic HPLC columns, imunosorbents, AAS and tandem multi-detector systems, for improving the analytical characterization of the reaction products. A better understanding of LCM oxidation and new information should be achieved supporting better control of the oxidation processes, better utilization of multi-component oxidants and catalysts in wood processing and subsequently further improvement of the environmental quality of technologies and properties of the final products.
VEGA 1/0070/03
New chromatographic methods of sampling and on-line analysis of chiral pollutants in air, water and soil - Milan Hutta, 2003-2005
New methods for the characterization and analysis of humic substances in soil and air dust particles were devised and published based on the proper use of a combination of step gradient, wide-pore octadecylsilica and solubilization power of dimethylformamide in a chromatographic system of HPLC with UV-VIS (DAD) and fluorescence detection. A study of the influence of co-sampled polen particles on various parameters of a new HPLC method for the determination of air humic-like substances (HULIS) was done. For diagnostic purposes a fast (3 min) HPLC method of analysis of urine porphyrins (including their positional isomers) at ultratrace (ng/l) level using monolithic column was designed and published. Interactions between analyte and the soil matrix were studied and on this basis a new approach to large-volume injection (20 ml and more) of soil extracts in RP-HPLC was devised, published and applied to the detection of pesticides (including chiral pyrethroids) at trace levels.
KEGA 3/1164/03
Internet and Computer Aided Distance Education of Analytical Chemistry Methods - Milan Hutta, 2003-2005
The project is devoted to the Internet and Computer Aided Distance Education of Analytical Chemistry Methods, course preparation and its implementation within the frame of long-term education programs for specialists of environmental and/or health control laboratories. The definitive goal was to take advantage of all current possibilities and properties of the Internet as a specific wide scope communication space and potential source of new educational tools. Information and resources shared via Internet, e.g. hypertext textbooks, processes and/or methods simulations, and other e-learning tools (teach-ware) enable the effective spread of new knowledge, including new theoretical and practical aspects of analytical methods. The project comprised the development of new tools for the education of both theoretical and practical aspects of Analytical Chemistry Methods, taught with simulation software e.g. HPLCSIM, MEKCSM, CESIM, GCSIM, newly developed Monolithic HPLCSIM etc.
KEGA 3/3116/05
School teaching of basic chemical laboratory techniques and safety at work in laboratory with assistance of visualization and motivation with utilization of possibilities of PC technique and internet - Radoslav Halko, 2005-2006
KEGA 3/3075/05
Virtual laboratory and simulated experiments as a tool for increase of effectiveness of education of chemical and energy-related subjects at natural sciences and technical universities, Miloslav Foltin, 2005-2007
KEGA 3/3176/05
Chemical reactions and equilibrium in analytical processes - Mária Chalányová, 2005
The project was focused on the elaboration of an e-learning CD-ROM based textbook on "Laboratory practice chemical reactions and equilibrium in analytical processes". The textbook contains a number of different analytical topics such as acid and base equilibrium, complex equilibrium, kinetic methods in quantitative chemical analysis, elemental analysis and determination of functional groups in organic analysis.
DAAD 13/2004
Development and study of New Analytical Methods for elemental speciation and preconcentration of metals in environmental samples, Institute of Chemistry, Department of Instrumental Analytics, University Duisburg-Essen, Germany - Mária Žemberyová, 2005-2006
Elaboration and study of new analytical procedures for preconcentration and speciation of Be, Mo, V and Cr in water samples for the determination by atomic spectrometry methods. Inorganic speciation of Mo, V, Cr in water samples in regard to differentiaton of oxidation states, using SPE preconcentration of the species. Study of the determination of bioavailable and specific forms using recommended (BCR) single and sequential procedures.
UK/266/2005
HPLC large volume injection of pesticides - Zoltán Hajdú, 2005
In this work we compared two approaches to injection of a sample in HPLC (large volume injection LVI and a "normal" volume injection directly onto the analytical column). The advantages and disadvantages of LVI HPLC applied to analysis of pyrethroid insecticides were evaluated. The method of direct LVI onto HPLC analytical column is applicable for the analysis of pyrethroides at low concentration levels. With the injection of 10 ml of soil extract of pyrethroids we were able to determine kadethrin with a concentration of 62,7 ng/ml, cypermethrin 56 ng/ml, trans - permethrin 37 ng/ml and cis - permethrin 13 ng/ml, contrary to injection of 50 l which abled determination of kadethrin with a concentration of 4 g/ml, cypermethrin 6 g/ml , trans - permethrin 5.87 g/ml and cis - permethrin 2 g/ml. The analysis was highly acceptable in spite of the fact that the sorption of these compounds to soil is significant.
UK/263/2005
New on-line chromatografic methods for pretreatment and detection of environmental samples and their speciation - Radoslav Halko, 2005
UK 307/2005
Preparative capillary isotachophoresis as a sample pretreatment technique in the analysis of cytosol - Róbert Bodor, 2005
The project deals with the use of preparative capillary isotachophoresis (pITP), operating in discontinuous fractionation mode, to the pretreatment of multi-component biosample - cytosol of mycobacterium smegmatis (MSmeg) before their analysis by other analytical methods. Preparative ITP was used in two sample pretreatment modes: 1) the isolation of trace analyte from the sample; 2) the fractionation of the sample into a certain number of fractions.The fractions of the samples obtained by preparative ITP were monitored by capillary isotachophoresis, by on-line combination of capillary zone electrophoresis with isotachophoresis (ITP-CZE) and by planar gel electrophoresis (SDS-PAGE). The off-line combination of the preparative ITP with other electrophoretic methods (ITP-CZE, planar gel electrophoresis) shows that pre-treatment of samples by pITP can be effective in better general separations of the proteinic samples. The results obtained in SDS-PAGE experiments shows that ITP offers orthogonal separating conditions to the molecular size based on separation of proteins by SDS-PAGE.
UK 265/2005
Two-dimensional electroseparations on the chip for trace analysis of ionogenic compounds in ionogenic matrices - Marián Masár, 2005
This work deals with the determination of chloride, sulfate and nitrate in drinking water by zone electrophoresis (ZE) on a column-coupling (CC) chip with conductivity detection. A low pH of the carrier electrolyte used in ZE separations suppressed potential disturbances of the co-migrating constituents, e.g., some inorganic anions and organic acids. The required differences in the effective mobilities of the analytes were reached by combining the ionic strength effect of bis-tris propane and a host-guest complex forming equilibria with ?-cyclodextrin or an association equilibria with 3-(N,N-dimethyldodecyl-ammonio)propane sulfonate. Under our working conditions, studied analytes were detected at a concentration of 100 ?g/l. Total analysis time below 450 s and a working range for chloride and sulfate up to 60 mg/l and for nitrate of up to 20 mg/l can be achieved via this method without sample dilution. Filtration and degassing were the only sample pre-treatment procedures. The use of an internal standard enabled an easy chip-to-chip and equipment-to-equipment transfer of the employed analytical method.
UK 103/2005
Direct Analysis of Solid Environmental Samples by Electrothermal Atomic Absorption Spectrometry - Ingrid Hagarová, 2005
From analytical techniques, electrothermal atomic absorption spectrometry (ETAAS) has been shown to be the most attractive technique for the direct analysis of solid samples. There are two well-established procedures for the direct analysis of solids by ETAAS, namely slurry sampling and solid sampling. The aim of this project was to develop simple and reliable procedures for direct determination of beryllium, molybdenum and vanadium in solid environmental samples (soils, sedimets, sludges) by slurry sampling ETAAS and solid sampling ETAAS.
